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. 2017 May 1;144(9):1698–1711. doi: 10.1242/dev.142042

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — Atoh7 expression is not affected by the combined deletion of Dlx1/Dlx2 and Brn3b. (A) In situ hybridization at E13.5 shows Atoh7 expression throughout the proliferating zone of developing retina, except for the ciliary margin. No difference was detected between wild type and the genetic mutants. Scale bar: 40 μm. (B) DLX2 and BRN3B expression is downregulated in Atoh7-null retinas at E13.5. (a-f) DLX2 expression is significantly reduced in the Atoh7 KO at E13.5 (a,b,e) but not at E16.5 (c,d,f). (G-J) BRN3B levels are severely reduced in the Atoh7^−/− retina at both E13.5 (g,h) and E16.5 (i,j).