Fig. 3.

BRC1 transcript levels respond to apical auxin. (A) Experimental setup: explants bearing single nodes were decapitated and 1 mM NAA (1-naphthylene acetic acid, a synthetic auxin) in lanolin or the control treatment was placed on decapitated stump at either 0 h or 3 h post-decapitation. Buds were collected 6 or 21 h post-decapitation. (B,C) BRC1 transcript levels in pooled bud samples of 15-20 explants treated according to A collected at 6 h post-decapitation (B) or 21 h post-decapitation (C). Brackets indicate the results of two-sample t-tests as not significant (n.s.) or significant at **P<0.05. (D) Bud length over time for each treatment, n=14-16. The data are representative of three independent biological repeats, error bars are s.e.m.