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. 2017 May 1;144(9):1725–1734. doi: 10.1242/dev.146837

Fig. 6.

Fig. 6.

Planar polarity is actively remodeled in moving cell populations. (A) Transverse edges that rotate into a vertical orientation as a result of cell rearrangements could retain Par-3 (model 1) or lose Par-3 and recruit myosin (model 2). (B) Stills of three Par-3-positive transverse edges (green) that rotate into a vertical orientation and accumulate myosin II (red). Time (min) relative to the start of edge rotation. Scale bar: 5 μm. (C) Par-3:GFP and myosin:mCherry intensity at edges that rotate from <45° to >70° (0° is parallel to the AP axis). Edge intensities were normalized to the mean intensity of all edges at that time point. A single mean value was obtained for each embryo and the mean±s.e.m. between embryos is shown. Time relative to the edge passing 70° (t=0, gray dashed line). Par-3 levels decreased (green bar) before myosin II levels increased (magenta bar). n=4 Par-3:GFP; myosin:mCherry movies; 89-240 rotating edges/movie. Vertical bars are guides to the eye. (D) Similar results were obtained with the fluorophores reversed. n=4 Par-3:mCherry; myosin:GFP movies; 19-54 rotating edges/movie.