Table 1.
Metabolic profiling of wild-type and transgenic A. thaliana lines as monitored by extracted ion chromatography from LC-MS (Fig. 2)
| No. | Metabolite | rt, min | [M+Na]+ | M+ | 1x | 2x | 3x | wt |
|---|---|---|---|---|---|---|---|---|
| 1 | p-glucosyloxy-benzylglucose* | 2.5 | 485 | 462 | - | + | - | - |
| 3.4 | 485 | 462 | - | + | - | - | ||
| 4.1 | 485 | 462 | - | ++ | - | - | ||
| 2 | p-glucosyloxy-phenylmethanol* | 11.7 | 309 | 286 | - | + | - | - |
| 3 | p-glucosyloxy-benzoic acid* | 5.8 | 323 | 300 | - | + | - | - |
| 4 | p-glucosyloxy-phenylethanol† | 10.3 | 323 | 300 | + | + | - | - |
| 5 | p-hydroxybenzoylglucose* | 11.1 | 323 | 300 | - | +++ | + | - |
| 6 | Dhurrin* | 13.5 | 334 | 311 | - | - | +++ | - |
| 7 | p-hydroxybenzylglucosionolate† | 4.0 | 368 | 345 | +++ | + | - | - |
| 8 | Desulfo p-hydroxybenzylglucosinolate† | 6.5 | 368 | 345 | +++ | + | - | - |
| 9 | Unknown glucoside | 12.0 | 337 | 314 | ++ | + | + | - |
| 10 | Unknown compound | 14.2 | - | 323 | + | + | - | - |
| 11 | Unknown compound | 7.7 | - | 323 | - | + | - | - |
| 12 | Unknown | 13.7 | 316 | +++ | + | +++ | +++ | |
| Unknown | 14.3 | - | 316 | +++ | ++ | +++ | +++ | |
| Unknown | 15.5 | 316 | +++ | ++++ | +++ | +++ | ||
| 13 | Sinapoyl malate | 23.2 | 363 | 339 | +++ | + | +++ | +++ |
| 14 | Sinapoyl glucose | 18.4 | 409 | 385 | + | + | +++ | +++ |
| 15 | Kaempferol-3-O-glucoside-7-O-rhamnoside | 22.0 | 617 | 594 | ++ | + | ++ | ++ |
| 16 | Kaempferol-3-O-rhamnoside-7-O-rhamnoside | 23.6 | 601 | 578 | ++ | + | ++ | ++ |
| 17 | Kaempferol-3-O-[rhamnosyl(1→2)glucoside]-7-O-rhamnoside | 19.7 | 763 | 740 | ++ | + | ++ | ++ |
+, Metabolite present with relative amounts indicated by the number of plus signs. -, metabolite was not detected; rt, retention time.
*
Metabolites marked derived from p-hydroxyphenylacetaldoxime.
†
Metabolites marked derived from p-hydroxymandelonitrile (6).