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. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: Bioorg Med Chem. 2017 Mar 4;25(8):2437–2444. doi: 10.1016/j.bmc.2017.02.066

Figure 2. Effects of the bishomoisoprenoid triazole bisphosphonates on protein geranylgeranylation.

Figure 2

RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control). B) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. C) Immunoblot analysis of the detergent fraction from Triton X-114 lysis of Rab6 and calnexin (loading control). Densitometric analysis of Rab6 (normalized to calnexin) for the treated cells normalized to untreated (control) cells is shown (relative intensity, Rel Int).