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. Author manuscript; available in PMC: 2017 May 31.
Published in final edited form as: Nature. 2016 Feb 17;530(7591):495–498. doi: 10.1038/nature16970

Fig. 1. Mud localizes at TCJ.

Fig. 1

(a) GFP:Mud from interphase to telophase (t=0 min, anaphase). GFP:Mud at TCJ (arrows), spindle poles (arrowheads). n=21 cells.

(b) GFP:Mud and Gli co-localization in interphase (top, n=54 cells) and metaphase (bottom, n=8 cells).

(c) GFP:Mud localization in mud (n=15), pins (n=22), Gαi (n=5) cells and GFP:MudΔPINS in mud cells (n=18).

(d,e) GFP:Mud distribution (d, images representative of quantifications shown in e) and mean±s.e.m. TCJ intensities (e) in wt, Gli, dlg and pins cells. Fas3, cell contours. t-test (ns: not significant, ***:p<0.0005). n: cell numbers.

Scale bars: 1μm (a, b, c, d).