Table 1.
Primers and probes used for multiplex real-time PCR to detect Cryptosporidium and Cyclospora simultaneously in vegetables examined from 2014 to 2015
| Parasites | Nucleotide position | Sequences (5′→3′) | Size (bp) | |
|---|---|---|---|---|
| Cryptosporidium | Forward primer | 2,930–2,950 | CTCCCCAGGAAGACGAAATAA | 242 |
| Reverse primer | 3,171–3,148 | TTCAAGCTCTCTTTCAATTTGCTC | ||
| Probe-FAM | 3,013–2,987 | AGCAAACAGGGCATCCAAGAACTCCTC | ||
|
| ||||
| Cyclospora | Forward primer | 918–940 | GCAGTCACAGGAGGCATATATCC | 116 |
| Reverse primer | 1,033–1,012 | ATGAGAGACCTCACAGCCAAAC | ||
| Probe-HEX | 1,005–981 | CGACGAACAGCCACGCACGCACTTG | ||