Figure 2.

Up-regulation of matrix metalloproteinase (MMP)19 in idiopathic pulmonary fibrosis (IPF) lungs. (A, B) Intense intracytoplasmic MMP19 labeling is observed in alveolar epithelial cells in IPF lungs (A, 40×; B, 10×). (C) Normal histology lungs showing no staining (40×). (D) IPF lung negative control omitting the primary antibody (40×). (E) ELISA assay of MMP19 protein in bronchoalveolar lavage of IPF lungs and normal volunteer lungs. (F) Western blot analysis of MMP19 in lung homogenates from IPF and normal lungs. (G) Densitometry of Western blot of MMP19 relative to β-actin. Data in E and G represent the mean ± SEM. NL = normal lungs.