Fig 1. Assembly statistics for the genome of Gopherus agassizii and comparison with Python bivittatus and Alligator mississippiensis.

(A) Two k-mer frequency peaks exist for G. agassizii: a peak at 35X coverage, representing heterozygous regions and a peak at 71X coverage, representing homozygous regions. (B) Gene-based scaffolding greatly increased scaffold N50 from 169.2 kbp to 251.6 kbp, which places it within the range of recently published genomes of reptiles including the Burmese python [71] and the American alligator [72].