Table 2. Median gene length, GC contents, number of transcripts, number of exons, exon length and intron length for essential and viable genes.

The median value of each feature is reported. p–values are determined from a Mann–Whitney U test. Statistically significant results were evaluated based on the Bonferroni corrected p–value of 0.0083.

Datasets			Gene Sequence Features
		Gene Length (bp)	GC content (%)	No. of transcripts	No. of exons	Exon length (bp)	Intron length (bp)
Non-culled	Essential	28913	46.46	4	11	3398	25341
	Viable	21629	47.16	2	8	2780	18563
	p-value	7.9×10−8	0.009	4.7×10−16	9.4×10−16	1.2×10−22	2.0×10−6
Culled (20%)	Essential	22757.5	46.34	4	10	2831	19226
	Viable	15931	46.82	2	7	2263	13761
	p-value	5.0×10−6	0.091	1.3×10−10	6.3×10−9	2.2×10−10	5.8×10−5
Culled (40%)	Essential	28548	46.21	4	11	3368	24928
	Viable	20280	46.98	2	8	2632	17667
	p-value	1.4×10−8	0.009	1.8×10−15	1.3×10−20	9.1×10−23	3.3×10−7
Culled (60%)	Essential	29601	46.22	4	11	3409	25964
	Viable	21267	47.08	2	8	2746	18310
	p-value	1.4×10−9	0.001	4.5×10−17	2.2×10−18	2.7×10−24	4.1×10−8
Culled (80%)	Essential	28936	46.45	4	11	3398	25333
	Viable	21574	47.15	2	8	2767	18534
	p-value	1.8×10−7	0.013	5.5×10−16	1.2×10−15	1.2×10−22	3.0×10−6