Table 6. Median values of different protein features obtained from Pepstats and the p-values of their distribution calculated using the Mann–Whitney U test.

The p-value for the Bonferroni correction is 0.0038.

Datasets		Protein Sequence Features
		Molecular weight (Da)	Aliphatic (%)	Aromatic (%)	Non-polar (%)	Polar (%)	Charged (%)	Basic (%)	Acidic (%)
Non-culled	Essential	59146	27.0	10.0	52.1	47.9	25.8	14.0	11.8
	Viable	50446	27.8	10.8	53.7	46.3	24.5	13.3	11.0
	p-value	3.4×10−21	3.5×10−13	3.7×10−14	4.4×10−27	4.6×10−27	2.3×10−18	2.2×10−15	1.8×10−13
Culled (20%)	Essential	48926	28.2	10.5	53.0	47.0	25.8	14.0	11.7
	Viable	40327	28.4	10.8	54.0	46.0	24.7	13.4	11.0
	p-value	4.1×10−10	0.4	0.2	1.0×10−4	1.0×10−4	7.6×10−5	4.9×10−4	6.4×10−4
Culled (40%)	Essential	60212	27.4	10.1	52.1	47.9	26.0	14.0	12.0
	Viable	48362	28.0	10.7	53.7	46.3	24.5	13.3	11.0
	p-value	3.2×10−21	6.0×10−6	4.1×10−9	1.6×10−20	1.6×10−20	3.7×10−19	5.6×10−12	3.4×10−16
Culled (60%)	Essential	60237	27.1	10.1	52.0	48.0	25.9	14.0	11.8
	Viable	50058	27.8	10.7	53.8	46.2	24.4	13.2	11.0
	p-value	1.4×10−21	3.4×10−11	2.4×10−12	4.9×10−28	5.1×10−28	1.9×10−21	1.9×10−15	4.6×10−17
Culled (80%)	Essential	59285	27.0	10.0	52.1	47.9	25.8	14.0	11.8
	Viable	50479	27.8	10.7	53.7	46.3	24.5	13.2	11.0
	p-value	1.2×10−20	1.2×10−12	1.4×10−14	1.0×10−27	1.1×10−27	3.9×10−19	8.6×10−16	3.4×10−14