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. 2017 May 31;12(5):e0178446. doi: 10.1371/journal.pone.0178446

Table 1. Primers used for cloning NcLPMO9C into pPpT4 expression vector.

Fragment Primer Sequence (5’ → 3’)
pPpT4_BB pPpT4_BB_fwd CATCAC*CATCAC*CATCACTAGGCGGCCGCTCAAGAG
pPpT4_BB_rev CGTTTC*GGAATT*CTTTCAATAATTAG
NcLPMO9C_gene NcLPMO9C_fwd AATTCC*GAAACG*ATGAAGACTGGTTCCATCTTGGCTGCTTTGGTTG
NcLPMO9C_rev GTGATG*GTGATG*TGGCAAACACTGGGAGTACCAGTC

BB = backbone; fwd = forward primer; rev = reverse primer;

* = phosphorothioate modification