Table 1. Primers used for cloning NcLPMO9C into pPpT4 expression vector.
Fragment | Primer | Sequence (5’ → 3’) |
---|---|---|
pPpT4_BB | pPpT4_BB_fwd | CATCAC*CATCAC*CATCACTAGGCGGCCGCTCAAGAG |
pPpT4_BB_rev | CGTTTC*GGAATT*CTTTCAATAATTAG | |
NcLPMO9C_gene | NcLPMO9C_fwd | AATTCC*GAAACG*ATGAAGACTGGTTCCATCTTGGCTGCTTTGGTTG |
NcLPMO9C_rev | GTGATG*GTGATG*TGGCAAACACTGGGAGTACCAGTC |
BB = backbone; fwd = forward primer; rev = reverse primer;
* = phosphorothioate modification