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. 2017 May 31;12(5):e0178515. doi: 10.1371/journal.pone.0178515

Fig 5. TPD52 regulates migration and invasion in CRC SW480 cells.

Fig 5

(A) SW480 cells were transfected with PG307-TPD52 overexpression vector (TPD52) or empty PG307 vector as negative control (Ctrl). Also, SW480 cells were transfected with siRNA for TPD52 (siTPD52) or control siRNA (siCtrl). Western blot analysis was used to detect TPD52 protein expression in cells using TPD52 antibody. GAPDH was detected as internal reference. (B,C) The cellular migration and invasion abilities were evaluated by Transwell assay. (D) Akt (Ser473) phosphorylation was detected by Western blotting using the corresponding antibody and Akt protein was examined. (E,F) TPD52-overexpressed SW480 cells were treated with 2 μM LY294002, the PI3K specific inhibitor, for 24 h, and migration and invasion abilities of TPD52-overexpressed and control cells were evaluated. *P<0.05, **P<0.01 compared with control cells; N.S., no significance.