Table 1. The structure of the oligonucleotides (direct primers) used to introduce mutations into a sequence of horse cytochrome c.

Primer	Oligonucleotides structure 5'-3'
T89A-dir	GTATTAAGAAGAAGGCTGAAAGAGAAG
T78S/K79P-dir	GAAGTACATTCCTGGTAGCCCGATGATTTTCGCTGG
I75G/G77R/T78I-dir	CCCAAAGAAGTACGGTCCTCGTATCAAGATGATTTTCGC
I81Y/A83Y/G84N-dir	CATTCCTGGTACTAAGATGTATTTCTACAATATTAAGAAGAAGACTGAAAG
K79V/I81L/F82R-dir	GTACATTCCTGGTACTGTGATGCTGCGCGCTGGTATTAAGAAG
I81L/F82S/A83S/G84A-dir	CTGGTACTAAGATGCTGTCCTCTGCTATTAAGAAGAAGAC
T78A/K79A/I81A/F82T-dir	GTACATTCCTGGTGCTGCGATGGCTACCGCTGGTATTAAG
T78N/K79Y/M80I/I81M/F82N-dir	CAAAGAAGTACATTCCTGGTAACTACATCATGAACGCTGGTATTAAGAAGAAGAC