Fig 1. Detection of DENV RNA and antibodies against DENV in the captured bats in the 3 sites of study in Costa Rica (Nicoya, Sarapiquí and Central Valley, S1 Fig) during the dry and rainy season, 2013–2014.

(A) Prevalence of DENV RNA obtained by PCR from each serotype of DENV in positive bat species. *Two individuals with joint detection of DENV-2 and DENV-4. **One individual with joint detection of DENV-2 and DENV-3. (B) Seroprevalence against each dengue serotype obtained from serum diluted 1:20 by PRNT₉₀ from positive bat species. Twelve individuals presenting antibodies against more than one serotype: † DENV-1 & DENV-3 (3) and DENV-2 & DENV-3 (4); † † DENV-1 & DENV-3 (2) and DENV-1 & DENV-4 (1); † † † DENV-1 & DENV-3 (2).