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. 2017 May 31;12(5):e0178624. doi: 10.1371/journal.pone.0178624

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© 2017 Tan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A, Expression patterns of endothelial makers on EPCs were analyzed by FACS. EPCs highly expressed CD31, vWF, CD144 and CD105, partly positive expressed VEGFR-2 and CD34, negative for CD90, CD45, CD14 and CD19. B, Biological function of EPCs was identified. a, Representative phase contrast images of cobblestone-like EPCs. b, EPCs bound with UEA-1 (red). c, EPCs incorporated DiI-Ac-LDL (green). d, EPCs formed vascular-like tubes on matrigel. C, Phenotype analysis of AD-MSCs and UC-MSCs by FACS. Both AD-MSCs and UC-MSCs were positive for CD29, CD90, CD73 and CD105, negative for VEGFR-2, CD14, CD31, CD34 and CD45. D, After 14 days of induction, AD-MSCs and UC-MSCs were differentiated into adipocytes and osteocytes. a, Adipogenic induction of AD-MSCs. b, Adipogenic induction of UC-MSCs. c, Osteogenic induction of AD-MSCs. d, Osteogenic induction of UC-MSCs. Adipogenesis was detected by the formation of neutral lipid vacuoles stainable with oil red O (red-orange). Osteogenesis was demonstrated by detection of alkaline phosphatase activity (brown).