Table 2. Methods to quantify asymmetry.
| Author reports asymmetry | Author reports no asymmetry | |||||
|---|---|---|---|---|---|---|
| Marker | Quantitative Threshold Based On: |
Marker | Quantitative Threshold Based On: |
|||
| Ratio of pixels between daughter cells | % of cells displaying asymmetry |
Ratio of pixels between daughter cells | % of cells displaying asymmetry |
|||
| Anti-4D2 | > 1 | No | NA | [35] | ||
| Dil18 | 1.92 ** | No | NA | [28] | ||
| Sec 61α | 1.2 ** | No | NA | [42] | ||
| SARA | 15 |
No | PtdIns(3)P, Rab5 | 3 |
No | [43] |
| SARA | 1.5 | 68% | Rab5c, Rab 7, Rab 11 | 1.5 |
7–9% | [45] |
| SARA | 4.81 | No | NA | [46] | ||
| SARA | 32 | No | Rab 7, Rab 11 | 1.9 | No | [47] |
| Oomp25 | 5.6 | No | Oomp25 | 1.25 | No | [22] |
| Mab48 | > 90% of one of two cell regions is devoid of labelling* | 0.8–11.5% | Mab48 | > 90% of one of two cell regions is devoid of labelling* | 0.1–0.33% |
[48] |
| Mito GFP | 3.5 ** | No | NA | [28] | ||
| PGL1 | 1.5 | No | NA | [49] | ||
| Proteoosome 20s | 1.5 | No | [32] | |||
* Assumes cell is dividing.
** fold enrichment either side of spindle.
1 Ratio based on reporting of 83% of fluorescence in mother cell goes to one daughter.
2 Ratio based on reporting of 75% of fluorescence in mother cell goes to one daughter.
Thresholds were not predefined, but were those reported y the author. If multiple thresholds were reported we selected the lowest (or range).