Fig. 3.

Assessment of the basement membrane proteins laminins and fibronectin. A) Quantitative proteomics for nine laminin subunits and fibronectin. Error bars are ± SEM, n = 4 biological replicates, with two different concentrations of QconCAT peptides. (Genes displayed in Supplemental Table 7.) Single asterisk (^*) indicates α = 0.05; (^**) indicates α = 0.01 (see Supplemental Table 5). ns = non-significant difference. Immunofluorescence for laminin γ1) in (B) native lung; (C) CHAPS lung; (D) Triton/SDC lung. Immunofluorescence for fibronectin in (E) native lung; (F) CHAPS lung; (G) Triton/SDC lung. Antigen of interest is stained in red. Nuclei are stained with DAPI (blue). Scale bars = 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)