Fig. 5.
Quantification of cellular components. Proteomic quantification of (A) high-abundance proteins and (B) low-abundance proteins in native and decellularized lungs. Error bars are ± SEM, n = 4 biological replicates, with two different concentrations of QconCAT peptides. (Genes displayed in Supplemental Table 7.) Single asterisk (*) indicates α = 0.05; (**) indicates α = 0.01 (see Supplemental Table 5). ns = non significant difference. C) Western blot for α-actin, MHC-1 and -2 molecules in native (lane 1) and decellularized lung samples. (Bleed-over is noted in lane 2, from the adjacent lane 1, in MHC-1 and -2 blots.) D) DNA quantification by PicoGreen Assay. Single asterisk (*) indicates statistically significant difference, p < 0.05; (**) indicates statistical significance, p < 0.01.