Fig. 5.

Distribution of MBs anterior to Descemet's membrane (DM) in normal (A–B) and keratoconic button 2 (C–D) cornea using orcein staining. Blue = DM, Gold = MBs, Pink/Purple = Keratocyte. Densely-stained MBs (white arrow) seen anterior to DM in normal cornea (A) were reconstructed, resulting in a 3D model (B) where the MBs were similar in number and orientation to those we have previously described in tannic acid stained cornea, with the majority travelling in one direction (yellow arrow). No MBs were identified in the keratoconic cornea data set, with poor contrast between DM and stroma (C). A keratocyte (blue arrow) was seen lying directly on top of DM (C–D), a finding that was seen frequently in other keratoconic data sets. Scale bars = 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)