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. 2017 May 1;10(5):581–595. doi: 10.1242/dmm.027433

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — anx/anx mice show deficits in secondary platelet aggregation. (A-C) The effect of (A) +/+, (B) anx/+ and (C) anx/anx platelets on CD62 (P-selectin) expression, a marker for α-granule secretion, was detected using flow cytometry. After activation with thrombin, CD62 levels on the platelet surface were significantly reduced in (C) anx/anx platelets. Black lines represent resting platelets and red shading denotes thrombin-stimulated platelets. Representative examples of at least four independent experiments are shown. (D) The percentage of secondary platelet aggregation in +/+ and anx/+ animals was 56.4±3.2% (n=17) and anx/anx animals 23.9±9.8% (n=6) (Mann–Whitney Test, P=0.026). Data represented as mean±s.e.m. (E,F) Electron microscopy analyses revealed that (F) anx/anx platelets appeared normal, but often contain large vacuoles (indicated by asterisks) absent in (E) +/+ platelets. The abnormal vacuoles were observed in 17.5±5.9% of anx/anx platelets (counted 57-63 platelets per replicate (n=4).