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. 2017 May 1;10(5):581–595. doi: 10.1242/dmm.027433

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — anx/anx lethality is delayed by Tyro3 transgenes, but not by an R7W-Tyro3 transgene. (A) Tyro3 transgenes were constructed using a 9.5 kb BamHI-NotI fragment upstream of the translational start site located in exon 2c (adapted from Biesecker et al., 1995). The signal sequence of TYRO3 is indicated in black. (B) The T3Xpressn vector was used to generate transgenic mice expressing mouse Tyro3-GFP, R7W-Tyro3-GFP and human TYRO3 (HuTyro3). The number of independent founder lines generated and analyzed is shown for each transgenic construct. (C) Analyses of survival of transgenic and non-transgenic anx/anx progeny. All anx/anx homozygous mice die by P21. In all mouse lines transgenic for Tyro3-GFP or huTyro3, transgenic anx/anx progeny survived past P35. anx/anx mice transgenic for R7W-Tyro3-GFP did not survive past P21. (D-F) Effect of Tyro3 transgenes on bodyweight. For (D) Tyro3-GFP line #1, (E) HuTyro3 line #1, and (F) the sum total of all HuTyro3 and Tyro3-GFP transgenic lines, transgenic anx/anx mice weigh more than non-transgenic littermates at P21. Groups with significant differences relative to each other are indicated with brackets and a single asterisk for anx/anx versus anx/anx; Tyro3 transgenic; # for +/+ and anx/+ vs anx/anx, and ^ for +/+ and anx/+ vs anx/anx; Tyro3 transgenic. P<0.01 for all groups by unpaired t-one-way ANOVA data represented as mean±s.e.m.