Fig. 5.

Rescue of Npy+ neuron abnormalities of anx/anx mice at P19. Npy+ neuronal abnormalities are ameliorated in Tyro3-GFP; anx/anx transgenic mice as detected by immunoperoxidase enhanced with silver staining with an anti-Npy antibody, which stains Npy+ neurons and processes black. (A,D) In P19 +/+ mice, Npy+ neurons are located in the arcuate nucleus (arc) and send processes to the paraventricular nucleus. A monolayer of Npy+ cells also lines the inside and outside of the median eminence (ME). (B,E) In P19 anx/anx mice, only a few Npy+ neurons are present in the arcuate nucleus, and Npy+ processes extending towards the PVN cannot be readily detected. In addition, some Npy+ cells are located abnormally in the median eminence (ME). (C,F) In Tyro3-GFP transgenic anx/anx mice, Npy+ neurons are mostly distributed throughout the arcuate nucleus and Npy+ puncta can be detected extending towards the PVN. D-F show higher magnification views of A-C, respectively. Dotted line in A-C demarcates the upper limit of the Npy+ soma included in the counts. (G) For anx/anx and anx/anx; Tyro3-GFP mice the percentage of Npy+ soma within the arcuate nucleus at the level of the median eminence relative to wild-type littermates are shown. Mice carrying Tyro3-GFP transgene (line #1) were analyzed. Cell counts were obtained from three sections with highest number of Npy+ neurons per animal and n=3 animals per genotype. Pairwise comparisons were performed using the Wilcoxon signed rank test; P<0.05.