Fig. 2.

Grb2 is upregulated and cytoskeletal proteins are downregulated in an AD-mimicking neuroblastoma cell model. (A)(i) Western blot (n=3) showing alterations of Grb2 and GAPDH levels in AICD-transfected, Aβ-treated and both AICD-transfected and Aβ-treated cells compared with respective negative controls (empty vector or solvent used for reagent). (ii) Graphical representation of the normalized variation of Grb2 expression compared to control cells (set as 1) (transfected with GFP, treated with DMSO and both transfected with GFP and treated with DMSO, respectively). (iii) Normalized fold changes of mRNA levels of Grb2 for qRT-PCR experiments (n=3), presented as a fold change relative to values in respective negative controls (empty vector or solvent used for reagents), with GAPDH taken as an internal control. (B) Western blots depict alterations in the expression of (i) α-tubulin, (ii) vimentin, (iii) α-smooth muscle actin (α-SMA) and (iv) stathmin 1 with GAPDH. The samples are derived from the same experiments and the blots were processed in parallel. (C) Histograms showing changes in the four cytoskeletal proteins respectively in (i-iv). Fold changes relative to controls are presented. (D) Shows transcript level changes for the four cytoskeletal proteins under AICD, Aβ and AICD+Aβ conditions with or without Grb2.