Fig. 5.

Accumulation of complexin 1 protein and downregulation of CPLX1 mRNA levels after overexpression of WT SNCA in SH-SY5Y cells. Human SH-SY5Y neuroblastoma cells were transiently transfected with plasmid pcDNA3.1(+) as an empty vector control or WT SNCA. (A) Immunoblot confirmation of successful SNCA overexpression and of the effect on complexin 1 protein levels on the second day after transfection, using GAPDH and β-actin as the loading controls (n=3 versus n=3 versus n=3 in independent experiments). (B) Densitometric quantification of complexin 1 (antibody from Synaptic systems) versus ACTB ratios, normalized against control, in a scattergram analysis with t-test. (C) Analysis of corresponding CPLX1 mRNA levels at 2 days after transfection. Asterisks indicate statistical significance versus control group (n=6 versus n=3 versus n=12). *P<0.05, **P<0.01. ACTB, β-actin; α-Syn, α-synuclein; WT, wild type.