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. 2017 May 6;6:e26120. doi: 10.7554/eLife.26120

Figure 5. Chromosome over-compaction depends on sister-chromatid proximity.

Figure 5.

(a) Stills from metaphase-arrested embryos after injection of TEV protease in strains surviving solely on Rad21TEV (cohesin cleavage) or Rad21TEV+BarrenTEV (cohesin and condensin cleavage); embryos also express HisH2B-RFP; scale bars, 5 μm. Insets show higher magnifications (3x) of single chromatids 20 min after TEV injection. Times (minutes:seconds) are relative to the time of TEV injection. (b–c) Relative frequency of sister chromatid length (b) and width (c) at 20 min after TEV injections (n ≥ 120 single chromatids from seven independent embryos for each experimental condition). (d) Mean voxel intensity of isolated single chromatids 20 min after TEV injections, normalized to mean voxel intensity 5 min past injection. (n ≥ 10 embryos for each experimental condition).

DOI: http://dx.doi.org/10.7554/eLife.26120.017

Figure 5—source data 1. Measurements of isolated chromatids upon cohesin/condensin inactivation.
Individual measurements of chromosome thickness, length and mean voxel intensity upon TEV-mediated cleavage of Read21TEV and Rad21TEV+BarrenTEV. Each data set is presented on a separate sheet. File includes descriptive statistics.
DOI: http://dx.doi.org/10.7554/eLife.26120.018
elife-26120-fig5-data1.xlsx (46.4KB, xlsx)
DOI: 10.7554/eLife.26120.018