Video 6. Concomitant inactivation of Topoisomerase II and Condenin I in metaphase-arrested embryos.
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DOI: 10.7554/eLife.26120.014
Embryos expressing solely BarrenTEV were injected with 12 mg/ml of a dominant-negative form of the human E2 ubiquitin-conjugating enzyme (UbcH10C114S), to induce a metaphase arrest, and subsequently injected with a mix of 280 μM ICRF-193 and 13 mg/ml TEV protease. Embryos also express His2A–mRFP1 (red) and Cid-EGFP (green); scale bars, 10 μm. Times (minutes:seconds) are relative to the time of the second injection.