Video 7. Artificial induction of sister chromatid separation in metaphase-arrested embryos.

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DOI: 10.7554/eLife.26120.019

Embryos expressing solely Rad21^TEV and wild-type Barren were injected with 12 mg/ml of a dominant-negative form of the human E2 ubiquitin-conjugating enzyme (UbcH10^C114S), to induce a metaphase arrest, and subsequently injected with 13 mg/ml TEV protease. Embryos also express His2B–RFP; scale bars, 10 μm. Times (minutes:seconds) are relative to the time of the second injection.

DOI: http://dx.doi.org/10.7554/eLife.26120.019