Video 8. Effect of condensin I inactivation on isolated sister chromatids.
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DOI: 10.7554/eLife.26120.020
Embryos expressing uniquely TEV-sensitive Rad21 and Barren were injected with 12 mg/ml of a dominant-negative form of the human E2 ubiquitin-conjugating enzyme (UbcH10C114S), to induce a metaphase arrest, and subsequently injected with 13 mg/ml TEV protease. Embryos also express His2B–RFP; scale bars, 10 μm. Times (minutes:seconds) are relative to the time of the second injection.