Table 2.
List of primers used. Location of primer sets with respect to transcription start-sites are indicated in brackets.
| Primer list | |
|---|---|
| Gene | Primers |
| eif4g2α | 5’-GAGATGTATGCCACTGATGAT |
| 5’-GCGCAGTAACATTCCTTTAG | |
| mxtx2 | 5’-ACTGACTGCATTGCTCAA |
| 5’-ACCATACCTGAATACGTGATT | |
| fam212aa | 5’-GCAAATGAGTATCTAAAACTGCT |
| 5’-CATCATATAGCGCATCTGGT | |
| nnr | 5’-GAGACATACCACAGGTGAAGC |
| 5’-CCGCTCTGGTCTGTTGC | |
| vox | 5’-TTATTCGTCGGGTTATGAGAG |
| 5’-AACCAAGTTCTGATCTGTGT | |
| sox19a | 5’-GAGGATGGACAGCTACGG |
| 5’-CTATAGGACATGGGGTTGTAG | |
| grhl3 | 5’-AGACGAGCAGAGAGTCCT |
| 5’-TTGCTGTAATGCTCGATGATG | |
| apoeb | 5’-GCAGAGAGCTTGACACACTAA |
| 5’-TGCATTCTGCTCCATCATGG | |
| dusp6 | 5'-AGCCATCAGCTTTATTGATGAG |
| 5'-CAAAGTCCAAGAGTTGACCC | |
| klf17 | 5’-ATAGTTCGGGACTGGAAAGTTG |
| 5’- TGAGGTGTTGTCGTTGTCAG | |
| irx7 | 5’-TGGCACACATTAGCAATTCC |
| 5’-GCATGATCTTCTCGCCTTTG | |
| klf2b | 5’-GCTCTGGGAGGATAGATGGA |
| 5’-CTCGGAGTGGGAGATGAAC | |
| flh | 5'-CACTGAAGCTCAGGTTAAAGTC |
| 5'-ACAATCTGGGGAAAATCATGG | |
| wnt11 | 5'-CAGACAGGTGCTTATGGACT |
| 5'-CATCTCTCGGGGCACAAG | |
| gadd45bb | 5'-CAACTCATGAATGTGGATCCAG |
| 5'-ATGCAGTGAAGGTCTCTTGG | |
| GFP | 5’-GCACCATCTTCTTCAAGGAC |
| 5’-TTGTCGGCCATGATATAGAC | |
| Pou5f3 binding site (−2270 apoeb) | 5'-TAAAGTGAGCAAATGTATGGCC |
| 5'-TTTGTTGATTAAATCGCTTGTGA | |
| Pou5f3 binding site (−3095 dusp6) | 5'-CATATGTTAAGCGGGGTGAAAC |
| 5'-ATCCTGTCTCCTGTGTCATTTG | |
| TRE binding site (−222) | 5'-TCTTGATAGAGAGGCTGCAAAT |
| 5'-TCGAGATGGGCCCTTGATA | |
| TRE binding site (13) | 5'-TCGTATAGGGATAACAGGGTAATG |
| 5'-TACACGCCTACCTCGACC | |
| TRE binding site (217) | 5'-GTACGGTGGGAGGCCTATAT |
| 5'-CTTCTATGGAGGTCAAAACAGC | |
| Transgene control | 5'-CTCTACAAATGTGGTATGGCTG |
| 5'-ATTACCCTGTTATCCCTAAGGC | |
| Genomic control | 5'-CCATCATATTCACATCTTGCAAG |
| 5'-GTTCGTATGAACCGGAAGC | |