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Midguts expressing GCaMP6s reporter together with trpA1 RNAi in ISCs were dissected and imaged in AHL buffer. Z-stack images were acquired with 10 s interval. A maximal intensity z-projection was shown in the movie. A final concentration of 0.03% TRPA1 agonist AITC was added at 150 s (the 15th frame of the movie).
