Figure 3. SDC-2 is required for open chromatin at strong DCC recruitment sites that encode for high intrinsic nucleosome occupancy.
(A) Heatmaps showing H3 ChIP-seq enrichment across a 1 kb window centered on the 64 recruitment sites. Wild-type data (left) indicates open chromatin at all recruitment sites. Data from an sdc-2 null strain (right) indicates increase in H3 enrichment, especially at the strong recruitment sites. Density plots above each heatmap indicate the average ChIP enrichment score for each recruitment class. (B) Heatmap indicates the probability of nucleosome occupancy across a 1 kb window centered on the recruitment sites. Probability ranges from 0.0 (yellow) to 1.0 (green). DNA-encoded nucleosome occupancy signal is highest at the center of the recruitment sites.