Figure 3. TTP ablation enhances neutrophil but not monocyte accumulation at the site of inflammation.

(A) Mice were injected i.p. with 2 × 10⁸ CFU HK S. pyogenes (n = 10 TTP^fl/fl and n =9 TTP^ΔM mice) or HK S. aureus (n = 8 TTP^fl/fl and n = 6 TTP^ΔM mice), or 2 × 10⁶ CFU HK E. coli (n = 4 TTP^fl/fl and n = 6 TTP^ΔM mice) 16 hours prior to cell collection. Dot plot shows total viable peritoneal cells. (B and C) Mice were challenged, and peritoneal cells were collected as in A. Representative flow plots (B) and total numbers (C) of neutrophils (Ly6G⁺Ly6C⁺) and inflammatory monocytes (Ly6G^–Ly6C^hi) obtained from challenged mice. Numbers in B represent the percentages of the outlined cell populations. Error bars indicate the mean. **P < 0.01 and ***P < 0.001, by unpaired Student’s t test.