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. Author manuscript; available in PMC: 2017 May 31.
Published in final edited form as: Cell Rep. 2017 Apr 11;19(2):321–334. doi: 10.1016/j.celrep.2017.03.056

Figure 6. STIM1- and LTCC-driven growth in spine ER content.

Figure 6

In neurons (12–14 DIV) expressing both RGECO1 and D1ER, 1 Hz uncaging of glutamate increased cross-sectional areas of spines and their ER compartment. (A) RGECO1 imaging of spine cross-sectional area.

(B) D1ER imaging of spine ER cross-sectional area.

(C) Quantitation of spine enlargement and growth in spine ER content in response to 1 Hz uncaging.

(D–F) Effects of antagonists of NMDARs (10 μM MK801) or of LTCCs (5 μM nimodipine) on uncaging-driven spine enlargement and growth in spine ER content. In F, the lines connecting filled and open symbols indicates that these measurements of spine and ER cross-sectional area were carried out in the same spine. Mean values in red.

(G–I) Effect on spine enlargement and growth in ER content of STIM1 knockdown, knockdown and rescue with hSTIM1, and knockdown and replacement with constitutively active hSTIM1(D76A).

Experimental data describing the growth in neighboring spine size is presented in Figure S7.