Fig. 9.4.
A bafilomycin clamp demonstrates BRAF inhibitor-induced autophagy. Immunoblotting against LC3 was performed using A375 (BRAF mutant melanoma) cell lysate 24 h after treatment as indicated. Representative image of one out three experiments is shown. Quantification of the bands demonstrates that in the presence of Baf A1, LC3II levels increased with PLX treatment compared no Baf A1