Fig. 3.

(a) Adsorption isotherms showing ubiquitin adsorption to MCM41 at 25 °C. Data recorded at pH 5.0 (green triangle), pH 7.5 (pink square), and pH 10.0 (blue diamond) are shown. Parameters from Langmuir-based fits (lines) are given in Table I. (b) ITC measurement of ubiquitin binding to MCM41 suspended in acetate buffer (pH = 5.0). (Top) Time-dependent trace of ITC measurement showing the heat released upon ubiquitin binding to MCM41. (Bottom) Integrated heat produced in each injection (▪) and fit of the titration to a single set of equivalent adsorption sites (solid line).