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. 2017 Jun;102(6):1017–1027. doi: 10.3324/haematol.2016.158717

Figure 4.

Figure 4.

Co-culture of double knockout mesenchymal stem/progenitor cells with hematopoietic stem/progenitor cells showed the decreased hematopoietic supportive activity. (A, B) DKO MSPC had reduced hematopoietic supportive activity as compared with WT MSPC (n=3 mice per genotype). Data are presented as mean ± SEM, *P<0.05, **P<0.01, ***P<0.001, two-way ANOVA followed by the Bonferroni test. (C, D) DKO MSPC supported HSC cultures contained higher percentages of the Gr1+/Mac1+ population and apoptotic CD45+ cells (n=3 mice per genotype). Data are presented as mean ± SEM, *P<0.05, **P<0.01, ***P<0.001, two-way ANOVA followed by the Bonferroni test. (E) MSPC-conditioned medium, adipocyte-conditioned medium, and the serum of DKO mice contained significantly increased concentrations of tumor necrosis-α (TNF-α) as compared with WT mice (n=3 mice per genotype). Data are presented as mean ± SEM, *P<0.05, **P<0.01, two-tailed Student t-test. (F) A marked reduction of the interleukin-6 (IL6) level was observed in DKO MSPC conditioned medium (n=3 mice per genotype). Data are presented as mean ± SEM, ***P<0.001, two-tailed Student t-test.