Figure 2.

Dynamics of the (pre)leukemic clone and non-malignant T-lymphocytes harboring the specific Vγ9-Jγ1.2 rearrangement during the pre-diagnostic phase. Quantitative levels of (pre)leukemic clones are connected by a solid line (left Y axis in each patient). Representations of the T-cell clone (defined by the specific Vγ9-Jγ1.2 rearrangement) within non-malignant cells are connected by a dashed line (right Y axis in each patient). Time points of sampling are shown on the X axis, relative quantification normalized to a housekeeping gene (ALB) is shown on the Y axis. Data are presented for individual patients (A–H). In patients UPN63 and UPN484 no biological material was available to analyze the T-lymphocytes of interest. All samples are from bone marrow except for the second pre-diagnostic sample from patient UPN1909 taken from peripheral blood (PB). D = dexamethasone treatment [(4 × 4 mg (UPN1071), 5 × 1 mg (UPN1909), 7 × 6 mg/m² (UPN_UK)].