Table 3.
Intensity ratios for 4 peptide markers for 6 test development set isolates.
| Isolate | KPC | Sample set | GFLAAAVLAR | LTLGSALAAPQR | APIVLAVYTR | LALEGLGVNGQa |
|---|---|---|---|---|---|---|
| ECNIH2 | + | A | 3.47 | 2.16 | 2.89 | 1.35 |
| ECNIH2 | + | B | 3.06 | 1.67 | 2.61 | 1.12 |
| ECNIH2 | + | C | 3.09 | 1.69 | 2.52 | 1.13 |
| ECNIH3 | + | A | 1.11 | 0.70 | 1.03 | 0.54 |
| ECNIH3 | + | B | 1.24 | 0.65 | 0.99 | 0.49 |
| ECNIH3 | + | C | 1.14 | 0.61 | 0.91 | 0.48 |
| E. coli | − | A | 0.03 | 0.00 | 0.02 | 0.11 |
| E. coli | − | B | 0.02 | 0.00 | 0.01 | 0.10 |
| E. coli | − | C | 0.02 | 0.00 | 0.02 | 0.11 |
| K. pneumoniae | − | A | 0.03 | 0.00 | 0.05 | 0.03 |
| K. pneumoniae | − | B | 0.03 | 0.00 | 0.05 | 0.03 |
| K. pneumoniae | − | C | 0.04 | 0.00 | 0.04 | 0.03 |
| KPNIH1 | + | A | 2.62 | 2.31 | 2.80 | 1.81 |
| KPNIH1 | + | B | 2.77 | 1.44 | 2.23 | 1.25 |
| KPNIH1 | + | C | 3.26 | 1.64 | 2.54 | 1.29 |
| KPNIH10 | + | A | 3.15 | 2.30 | 2.63 | 1.69 |
| KPNIH10 | + | B | 3.06 | 1.84 | 2.75 | 1.38 |
| KPNIH10 | + | C | 2.10 | 1.33 | 1.67 | 1.37 |
| Positive range | 1.11–3.47 | 0.61–2.31 | 0.91–2.89 | 0.48–1.81 | ||
| Negative range | 0.02–0.04 | <0.01 | 0.01–0.05 | 0.03–0.11 | ||
| Lowest Pos/Neg ratio | 27.8 | >61.0 | 18.2 | 4.4 |
Intensity ratio is calculated as signal peak intensity/daily positive control intensity. Technical triplicate samples (A, B, and C) were prepared at the same time. aBased on b-ions only due to N or Q deamination; this peptide was not used in validation.