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. 2017 May 31;7:2551. doi: 10.1038/s41598-017-02700-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Over-expression of GATA-1 protein level decreases IRF-3 promoter activity. (a) pcDNA or pcDNA-GATA-1 plasmid was co-transfected with pGL3-149 into cells. (b) IRF-3 protein level was declined by overexpressed GATA-1 in different lung adenocarcinoma cell lines. GAPDH was used as a loading control. Error bars represent the mean ± S.D. from three independent experiments (*p < 0.05; **p < 0.01). The full-length blots were presented in Supplementary Figure S4.