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. 2017 May 31;7:2504. doi: 10.1038/s41598-017-02701-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Urinary and plasma cotinine levels by genotype. Urinary cotinine (normalized for creatinine concentration) relative to plasma cotinine in Hhip ^+/− heterozygotes (left panel) and Hhip ^+/+ wild type mice (right panel) subjected to chronic cigarette smoke. In Hhip ^+/+ wild type mice, a strong inverse correlation between urine and plasma cotinine levels exist (Pearson rho = −0.89, p-value = 0.04) whereas in Hhip ^+/− heterozygotes, no correlation was found (Pearson rho = 0.12, p-value = 0.88). The best fit line is plotted in blue while the 95% confidence interval is plotted in dark gray.