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. 2017 May 31;7:2538. doi: 10.1038/s41598-017-02763-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic illustration of the driver constructs. All constructs contain the ZsGreen marker driven by the Lchsp83 gene promoter, a phiC31 attP site and 5′ and 3′ piggyBac ends. Expression of tTA is controlled by the Lsspt promoter in DR3 (A) and the Lcact5C promoter in DR5 (B). The Lcact5C promoter was also used to drive expression of the dtTomato (RFPto) reporter gene (C).