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. 2017 May 31;7:2520. doi: 10.1038/s41598-017-02782-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of a new miRNA signature for the assessment of colitis in a validation cohort of IL10^−/− mice. The expression levels of 13 deregulated miRNAs were validated by qPCR using a second and independent set of IL10^−/− mice. (A) Fold changes of the 12 expressed miRNAs in this new set of IL10^−/− colitic mice (D98) compared to IL10^−/− non-colitic mice (D28). The expression level of each miRNA was analyzed and standardized to 1 in non-colitic mice. (B) Hierarchical clustering showing the relative expression levels of the nine miRNAs in each sample. (C) Principal coordinate analysis (PCoA) plot of the euclidean distance matrix for miRNA expression in sera was performed using QIIME software and plotted using Emperor Software. Percentages on the axes indicate the percentage of variance explained by each component. The populations of the nine selected miRNAs in 28 day-old (blue) or 98 day-old (red) IL10^−/− mice were clustered using normed PCoA. Permanova test, p = 0.001. Data are presented as the means +/− S.E.M. (n = 9–18). Significance was determined using unpaired t-test (*p < 0.05; **p < 0.01; ***p < 0.001; NS, not significantly different) compared to the results obtained from the corresponding D28 IL10^−/− mice.