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. 2017 May 31;7:2549. doi: 10.1038/s41598-017-02352-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDF-1/CXCR4 axis is active on ECM cultured HSPCs. (a) Proportion of migrated fresh-, SN- and AT-cells in a trans-well migration assay towards SDF-1 containing medium (with SDF-1) or towards SDF-1 free medium (spontaneous migration, w/o SDF-1). n = 4, two-tailed t-test; * = significance in comparison to SN-cells and + = significance in comparison to corresponding w/o SDF-1. (b) Confocal microscopy images of CXCR4 (red) or SDF-1 (green) staining and nuclear DAPI (blue) staining. Cells were treated with medium either without (w/o) or with additional SDF-1. Schemas show technique and findings. Bars = 5 µm. (c) Confocal microscopy z-stack images of α-CXCR4 (red) and nuclei DAPI (blue) staining. A = top, B = middle and C = bottom of cells. Cells were treated with medium either without or with additional SDF-1. Schemas show technique and findings. Bars = 5 µm. (d) Proportion of cell adhesion to ECMs 24 hours after seeding either without or with additional SDF-1 containing medium. n = 6 for w/o additional SDF-1, n = 3 for with additional SDF-1, two-tailed t-test. Error bars, SD.; *p < 0.05, **p < 0.01, ***p < 0.001.