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. 2017 May 31;7:2549. doi: 10.1038/s41598-017-02352-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Integrins recognizing RGD-motives promotes active focal contact formation. (a) FACS analysis of freshly isolated cells or 5 days ECM or MSC co-cultured cells. Percent positive cells for ITGαIIb (CD41), ITGαV and ITGβ3 (CD61) are shown. n = 4, two-way ANOVA with Bonferroni post-hoc test; + = significance in comparison to fresh cells. Error bars, s.e.m.; *p < 0.05, **p < 0.01, ***p < 0.001. (b) Confocal microscopy images of α-ITGαVβ3 (left panel, yellow), α-vinculin (mid penal, red), α-p-paxillin (right panel, red), nuclei DAPI (blue) staining, merge and corresponding brightfield images of 5 days cultured AT-cells. Bars = 5 µm. (c) Confocal microscopy images of α-ITGαVβ3 (yellow), α-p-paxillin (right panel, red), nuclei DAPI (blue) staining and merge including corresponding brightfield image of 5 days cultured AT-cell. Arrowheads represent co-localization of ITGαVβ3 and p-paxillin in a cellular protrusion. Bar = 5 µm.