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. 2017 May 31;7:2549. doi: 10.1038/s41598-017-02352-5

Figure 6.

Figure 6

ECM scaffolds induce CD61 surface expression on HSPCs. (a) Representative gating strategy for fluorescence activated cell sorting experiments. Lymphocytes and single cells were enriched using light-scatter. Dead cell exclusion was done by DAPI staining. Progenitor enrichment was done using SSC-Alow/CD45dim and CD34+ gate. Sorting gates CD34+CD61- and CD34+CD61+. (b) Representative FACS plots of CD34+CD61+ and CD34+CD61- sorted fresh cells after 5 days in ECM culture. AT- and SN-cells are shown regarding CD34 and CD61 expression using gates according to sort experiments. (c) FACS analysis of CD34+CD61+ and CD34+CD61- sorted cells after 5 days in ECM culture to CD61 expression using gates according to sort experiments. n = 2, Error bars, SD.; *p < 0.05, **p < 0.01, ***p < 0.001. (d) Cell count of AT- and SN-cells of CD34+CD61+ and CD34+CD61- sorted fresh cells after 5 days in ECM culture. n = 2, * = significance compared whole CD34+CD61+ and CD34+CD61- pre-sorted population including AT- and SN-cells, two-tailed t-test; Error bars, SD.; *p < 0.05, **p < 0.01, ***p < 0.001.