Figure 3.

Structure activity relationships and selection of ChX710. (a) Chemical structures of ChX67779 and two analogs called CID11207170 and ChX0306710. Compounds were tested for their capacity to induce the ISRE-luciferase reporter gene at three different concentrations. (b and c) HEK-293T cells were left untreated (DMSO alone) or incubated with ChX710 at indicated concentrations for 0, 4, 16 or 24 hours. IFI27 (b) and IFI6 (c) induction levels were determined by RT-qPCR as described above. Experiments were performed in duplicate, and data represent means ± SD. *P < 0.05 and **P < 0.01 as calculated by two-way ANOVA with Bonferroni’s post hoc test. (d) Human PBMCs were treated for 24 hours with DMSO alone or ChX710 at 12.5 or 25 μM, and IFI27 inductions was determined by RT-qPCR as described above. Data correspond to four healthy donors depicted by different symbols. *P < 0.05 as calculated by one-way ANOVA with Bonferroni’s post hoc test.