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. 2017 May 31;7:2561. doi: 10.1038/s41598-017-02776-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ISRE induction by ChX710 is independent of IFN-α/β, STAT1, and STAT2. (a) ISRE-luciferase reporter cells were incubated, at 4x10⁴ cells/well in a 96-well plate, with DMSO alone or increasing concentrations of ChX710 in the presence of both blocking antibodies against IFN-α/β. After 24 hours of incubation, luciferase induction was determined. Experiment was performed in duplicate, and data represent means ± SD. NS indicates non-significant differences as calculated by two-way ANOVA. (b) STAT1, STAT2 or STAT1 and STAT2 were silenced by siRNA transfection in ISRE-luciferase reporter cells. After 48 hours of cultures, cells were stimulated with ChX710. After 24 hours of incubation, luciferase induction was determined. Data represent means ± SD of three independent experiments. NS indicates non-significant differences relative to control siRNA (CT) as calculated by two-way ANOVA.