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. 2017 May 31;7:2510. doi: 10.1038/s41598-017-02820-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Compounds: chemical structure of tested compounds. Dose-response: ER transcriptional activity measured as luciferase activity in the presence of increasing concentrations of indicated compounds in the ERE-Luc B17 cells, fold induction of luciferase activity measured at 10-5 M of indicated compound respect to vehicle. EC50: plotting of the transactivation data for EC50 calculation by means of sigmoidal dose-response (variable slope) using PRISM5 software.