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. 2017 May 31;7:2510. doi: 10.1038/s41598-017-02820-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of the wound areas after treatment with 1e and 17β-estradiol. (a) Wound area measurement: each bar represents the mean ratio versus time = 0 h +/− SEM expressed as percentage of wound area measured in 4 animals/group. (b) Representative staining of wound explants at 72 hours after treatments. Staining was done with Iba1 antibody. Pictures were taken at 25x and 400x. Bar = 2 mm. The number of macrophages cells around wound site: each bar represents the mean +/−SEM, macrophages (Iba1 positive cells) were counted by analyzing n = 6 fields (vehicle), n = 11 fields (1e) and n = 10 fields (17β-estradiol) at 400x enlargement. (*p < 0.1 vs vehicle, ANOVA followed by Bonferroni’s test for multiple comparisons).